Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants.
J Biosci
;
2002 Mar; 27(2): 105-12
Artículo
en Inglés
| IMSEAR
| ID: sea-111031
ABSTRACT
The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by the erg-3 and erg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurospora erg-3 and SR-1 protein sequences and tested them for complementation of the Neurospora erg-3 mutant. To our surprise, all the chimeras failed to complement erg-3. A few of the chimeric proteins were also tested against the yeast erg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Oxidorreductasas
/
Proteínas Recombinantes de Fusión
/
Humanos
/
Datos de Secuencia Molecular
/
Mutagénesis Sitio-Dirigida
/
Alineación de Secuencia
/
Secuencia de Aminoácidos
/
Oxidorreductasas actuantes sobre Donantes de Grupo CH-CH
/
Prueba de Complementación Genética
/
Animales
Idioma:
Inglés
Revista:
J Biosci
Año:
2002
Tipo del documento:
Artículo
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