Your browser doesn't support javascript.
loading
Regulation of activity of the yeast TATA-binding protein through intra-molecular interactions.
J Biosci ; 2003 Jun; 28(4): 413-21
Artículo en Inglés | IMSEAR | ID: sea-111099
ABSTRACT
Dimerization is proposed to be a regulatory mechanism for TATA-binding protein (TBP) activity both in vitro and in vivo. The reversible dimer-monomer transition of TBP is influenced by the buffer conditions in vitro. Using in vitro chemical cross-linking, we found yeast TBP (yTBP) to be largely monomeric in the presence of the divalent cation Mg2+, even at high salt concentrations. Apparent molecular mass of yTBP at high salt with Mg2+, run through a gel filtration column, was close to that of monomeric yTBP. Lowering the monovalent ionic concentration in the absence of Mg2+, resulted in dimerization of TBP. Effect of Mg2+ was seen at two different levels at higher TBP concentrations, it suppressed the TBP dimerization and at lower TBP levels, it helped keep TBP monomers in active conformation (competent for binding TATA box), resulting in enhanced TBP-TATA complex formation in the presence of increasing Mg2+. At both the levels, activity of the full-length TBP in the presence of Mg2+ was like that reported for the truncated C-terminal domain of TBP from which the N-terminus is removed. Therefore for full-length TBP, intra-molecular interactions can regulate its activity via a similar mechanism.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Unión Proteica / Conformación Proteica / Saccharomyces cerevisiae / Regulación Fúngica de la Expresión Génica / Cromatografía en Gel / TATA Box / Dimerización / Reactivos de Enlaces Cruzados / Proteína de Unión a TATA-Box / Relación Dosis-Respuesta a Droga Idioma: Inglés Revista: J Biosci Año: 2003 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Unión Proteica / Conformación Proteica / Saccharomyces cerevisiae / Regulación Fúngica de la Expresión Génica / Cromatografía en Gel / TATA Box / Dimerización / Reactivos de Enlaces Cruzados / Proteína de Unión a TATA-Box / Relación Dosis-Respuesta a Droga Idioma: Inglés Revista: J Biosci Año: 2003 Tipo del documento: Artículo