Defence transcriptome profiling of Zingiber zerumbet (L.) Smith by mRNA differential display.
J Biosci
;
2008 Mar; 33(1): 81-90
Artículo
en Inglés
| IMSEAR
| ID: sea-111198
ABSTRACT
Soft rot is a serious disease in ginger (Zingiber of?cinale Roscoe),imposing a considerable economic loss annually in all ginger-producing countries.In this study,mRNA differential display was employed to identify genes whose expression was altered in a soft rot-resistant accession of Zingiber zerumbet (L.) Smith,a wild relative of ginger, in response to Pythium aphanidermatum (Edson) Fitzp.,which is the principal causative agent of soft-rot disease in ginger.Analysis using 68 primer combinations identified 70 differentially expressed transcript-derived fragments (TDFs),of which 34 TDFs were selected for further analysis following reverse northern screening.Cloning and sequence characterization of the 34 TDFs yielded a total of 54 distinct clones.Functional categorization of these clones revealed seven categories,of which the defence/stress/signalling group was the largest,with clones homologous to genes known to be actively involved in various pathogenesis-related functions in other plant species.The significance of these genes in relation to the resistance response in Z.zerumbet is discussed.This study has provided a pool of candidate genes for detailed molecular dissection of the defence mechanisms in Z.zerumbet and for accessing wild genetic resources for the transgenic improvement of ginger.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Enfermedades de las Plantas
/
Pythium
/
Transcripción Genética
/
ARN Mensajero
/
Análisis de Secuencia de ADN
/
Genes de Plantas
/
Zingiber officinale
/
Perfilación de la Expresión Génica
Idioma:
Inglés
Revista:
J Biosci
Año:
2008
Tipo del documento:
Artículo
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