Aujeszky's disease virus production in disposable bioreactor.
J Biosci
;
2006 Sep; 31(3): 363-8
Artículo
en Inglés
| IMSEAR
| ID: sea-111219
ABSTRACT
A novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky's disease virus (ADV) production. Growth kinetics of BHK 21 C13 cells in the wave bioreactor during 3-day period were determined. At the end of the 3-day culture period and cell density of 1.82x10(6) cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain ADV suspension (10(5.9) TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of ADV harvest was obtained with titre of 10(7.0) TCID 50 ml-1, which corresponds to 40,000 doses of vaccine against AD. In conclusion, the results obtained with the wave bioreactor using BHK 21 C13 cells showed that this system can be considered as suitable for ADV or BHK 21 C13 cell biomass production.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Cultivo de Virus
/
Línea Celular
/
Cricetinae
/
Herpesvirus Suido 1
/
Biomasa
/
Técnicas de Cultivo de Célula
/
Reactores Biológicos
/
Equipos Desechables
/
Animales
Tipo de estudio:
Estudios de evaluación
Idioma:
Inglés
Revista:
J Biosci
Año:
2006
Tipo del documento:
Artículo
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