Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana.
J Biosci
;
2008 Mar; 33(1): 91-101
Artículo
en Inglés
| IMSEAR
| ID: sea-111306
ABSTRACT
A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP)from the leaves of Bougainvillea x buttiana was isolated.The cDNA consisted of 1364 nucleotides with an open reading frame (ORF)of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids.The deduced amino acid sequence has a putative active domain conserved in RIPs/AVPs and shows a varying phylogenetic relationship to the RIPs from other plant species.The deduced protein has been designated BBAP1 (Bougainvillea x buttiana antiviral protein1).The ORF was cloned into an expression vector and expressed in E.coli as a fusion protein of approximately 78 kDa.The cleaved and purified recombinant BBAP1 exhibited ribosome-inhibiting rRNA N-glycosidase activity,and imparted a high level of resistance against the tobacco mosaic virus (TMV).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Antivirales
/
Filogenia
/
Proteínas de Plantas
/
Virus del Mosaico del Tabaco
/
Proteínas Recombinantes de Fusión
/
Señales de Clasificación de Proteína
/
Datos de Secuencia Molecular
/
Inhibidores de la Síntesis de la Proteína
/
Secuencia de Bases
/
Expresión Génica
Idioma:
Inglés
Revista:
J Biosci
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS