Programmed cell death as a prognostic indicator for radiation therapy in cervical carcinoma patients: a pilot study.
J Cancer Res Ther
;
2005 Jan-Mar; 1(1): 41-5
Artículo
en Inglés
| IMSEAR
| ID: sea-111565
ABSTRACT
PURPOSE:
In clinical practice, radiation therapy often fails in cervical carcinoma stage IIIB and there is a need to develop a predictive assay for prognosis of radiation treatment outcome in cancer patient. We have attempted to evaluate the relevance of changes in Membrane Fluidity (MF) and associated apoptotic cell death in cervical cancer cells after first fractionated dose of radiation therapy to treatment outcome of stage IIIB cervical carcinoma patients. MATERIALS ANDMETHODS:
Biopsies of 15 patients with histologically proven cervix cancer were collected from the patients before and 24 h after first fractionated radiation dose of 2 grays (Gy). Cell suspension made in Dulbecco's Modified Eagle's Medium (DMEM) were used for further investigations and cell suspension of cervix cancer patient were used to measure MF by fluorescence polarization method and apoptotic index (AI) was determined by Tdt dUTP Nucleotide End Labeling (TUNEL) assay.RESULTS:
A substantial increase in MF and AI was observed in cervical cancer cells irradiated ex vivo . A significant correlation ( P P > 0.1) was detected between changes in MF and treatment outcome of patients.CONCLUSION:
Preliminary results showed significant change in MF and a marked increase in percentage apoptosis of cervix cancer cells irradiated ex vivo . The changes in AI after first fractionated dose of radiotherapy in cervical carcinoma patients may provide a predictor of prognosis for radiotherapy in uterine cervical carcinoma patients.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Pronóstico
/
Anciano
/
Femenino
/
Humanos
/
Neoplasias del Cuello Uterino
/
Proyectos Piloto
/
Resultado del Tratamiento
/
Apoptosis
/
Adulto
/
Etiquetado Corte-Fin in Situ
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
J Cancer Res Ther
Asunto de la revista:
Neoplasms
/
Therapeutics
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS