Identification of a Taq DNA polymerase inhibitor from the red seaweed Symphyocladia latiuscula.
J Environ Biol
;
2008 Jul; 29(4): 475-8
Artículo
en Inglés
| IMSEAR
| ID: sea-113629
ABSTRACT
Two inhibitors of Taq DNA polymerase were isolated from the marine red alga Symphyocladia latiuscula. The inhibitors were purified by methanol extraction, molecular fractionation below 3000 MW and reverse-phase HPLC. The purified compound SL-1 containing three bromines was identified as 2,3,6-tribromo-4,5-dihydroxybenzyl alcohol (C7H5Br3O3 MW374) by NMR and MS analyses. The purified compound SL-2 was identified as 2,3, 6-tribromo-4,5-dihydroxybenzyl methyl ether(C8H7Br3O3 MW388). In a 25-microl reaction mixture containing 1.5 units of Taq DNA polymerase, the enzyme was completely inhibited by 0.5 microg SL-1 or 5 microg SL-2.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Análisis Espectral
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Cromatografía Líquida de Alta Presión
/
Polimerasa Taq
/
Metanol
/
Rhodophyta
/
Electroforesis en Gel de Agar
/
Inhibidores Enzimáticos
/
Éteres
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
J Environ Biol
Año:
2008
Tipo del documento:
Artículo
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