Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development.
J Genet
;
2009 Apr; 88(1): 33-9
Artículo
en Inglés
| IMSEAR
| ID: sea-114423
ABSTRACT
The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 x fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male or female parent. We localized fmf-1 to the LG IL genome segment between the centromere-proximal breakpoint of the chromosome segment duplication Dp(IL)39311 and the centromere. By mapping crossovers with respect to RFLP markers in this region we further localized fmf-1 to an approximately 34-kb-genome segment. Partial sequencing of this segment revealed a point mutation in the gene NCU 09387.1, a homologue of the Schizosaccharomyces pombe ste11+ regulator of sexual development. The fmf-1 mutation did not complement a NCU 09387.1 deletion mutation, and transformation with wild-type NCU 09387.1 complemented fmf-1. S. pombe Ste11 protein (Ste11p) is a transcription factor required for sexual differentiation and for the expression of genes required for mating pheromone signalling in matP and matM cells. If FMF-1 also plays a corresponding role in mating pheromone signalling in Neurospora, then protoperithecia in an fmf-1 x fmf-1+ cross would be unable to either send or receive sexual differentiation signals and thus become arrested in development.
Texto completo:
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Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Schizosaccharomyces
/
Factores de Transcripción
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Proteínas Fúngicas
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Regulación Fúngica de la Expresión Génica
/
Genoma Fúngico
/
Proteínas de Schizosaccharomyces pombe
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Modelos Genéticos
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Mutación
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Neurospora crassa
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
J Genet
Año:
2009
Tipo del documento:
Artículo
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