Characterization of gene expression regulated by human OTK18 using Drosophila melanogaster as a model system for innate immunity.
J Genet
;
2008 Aug; 87(2): 109-17
Artículo
en Inglés
| IMSEAR
| ID: sea-114443
ABSTRACT
OTK18 is a human transcriptional suppressor implicated in the regulation of human immunodeficiency virus type-one infection of mononuclear phagocytes. It is ubiquitously expressed in all normal tissues, but its normal homeostatic function is yet to be characterized. One hypothesis is that OTK18 aids in the regulation of the innate immune system. To test this hypothesis, cDNA microarray analysis was performed on the total RNA extracted from Drosophila melanogaster embryonic Schneider 2 (S2) cells transfected with either pEGFP-OTK18 (enhanced green fluorescent protein) or empty vector controls (pEGFP-N3) for 6, 12 and 24 h. cDNA microarray analysis revealed differential expression of genes known to be important in regulation of Drosophila innate immunity. The expression levels of two genes, Metchnikowin and CG16708 were verified by quantitative real-time reverse transcription PCR. These results suggest a role for OTK18 in innate immunity.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Factores de Transcripción
/
Humanos
/
Transfección
/
Línea Celular
/
Regulación de la Expresión Génica
/
Reproducibilidad de los Resultados
/
ADN Complementario
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Análisis de Secuencia por Matrices de Oligonucleótidos
/
Perfilación de la Expresión Génica
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
J Genet
Año:
2008
Tipo del documento:
Artículo
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