Determination of pyrazinamide in human by high performance liquid chromatography.
J Postgrad Med
;
1994 Jan-Mar; 40(1): 7-9
Artículo
en Inglés
| IMSEAR
| ID: sea-116174
ABSTRACT
A facile and sensitive high performance liquid chromatographic (HPLC) technique has been developed for the determination pyrazinamide (PZA) in human plasma. Nicotinamide(NIA) is used as internal standard(IS). Plasma is deproteinized with 0.7 M perchloric acid; clear supernatant is neutralized with 1M NaOH and injected onto HPLC. The separation of pyrazinamide and the internal standard is carried out on a Supelco LC-18 (DB) column with a basic mobile phase. Pyrazinoic acid, the major metabolite, other anti-tuberculous drugs and endogenous components do not interfere with measurement of pyrazinamide. The limit of detection of pyrazinamide with this method is 0.2 mg/0.2 ml plasma (CV 8.2%).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Pirazinamida
/
Humanos
/
Masculino
/
Disponibilidad Biológica
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Niacinamida
/
Adulto
/
Antituberculosos
Idioma:
Inglés
Revista:
J Postgrad Med
Año:
1994
Tipo del documento:
Artículo
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