Some variation in membrane proteins of erythrocytes obtained from subjects with thalassaemia trait and G-6-PD deficient genes

ABSTRACT

A total of 350 erythrocyte samples were screened for haemoglobin variant, G-6-PD deficiency and thalassaemia trait genes. Of these 104 samples were subjected to membrane protein analysis using SDS-PAGE electrophoresis and double straining with silver and Coomassie Blue. Membrane protein patterns of erythrocytes from subjects with a single genetic defect (i.e. either G-6-PD deficient or thalassaemia trait) were found to be similar to that of normals but those from subjects with double genetic defects (i.e. combination of G-6-PD deficiency and thalassaemia trait) had a significantly reduced content of spectrin, band 3 and glycophorin A.