A histomorphometrical comparative study between the use of platelet rich plasma and e-PTFE membrane in bone regeneration of canine mandible: A pilot study

Platelet rich plasma (PRP) has been used as an accelerator in bone healing in implant dentistry with conflicting results. Aim of this study was to compare bone regeneration rate histomorphometrically, between the use of PRP and e- PTFE membrane in artificial defects of the canine mandible at different periods of healing. Four standardized artificial defects were prepared at the lower border of mandibles of ten dogs. One defect was filled with PRP, one was covered with e-PTFE membrane (Goretex®), one was filled with PRP and covered with e- PTFE membrane, and one defect served as control. Collagen (Tissue vlies®) was added to each defect. Samples were taken from two animals at 2, 4, 6, 8 and 12 weeks. Representative bone specimens were fixed in 10 % buffered formalin prior to histological preparation of ground sections. Digital images of sections were analyzed histomorphometrically for newly formed bone. Results were analysed statistically. Results at different healing periods showed that addition of PRP did not improve healing of bone significantly (p=0.53). The use of e-PTFE membrane improved bone healing after two weeks significantly (p