Measurement of urinary oestrogen level for monitoring of ovulation.

ABSTRACT

A new micrometering kit using latex agglutination inhibition reaction for determination of urinary oestrogen of 15 infertile women undergoing induction with the injections of HMG – HCG (human menopausal gonadotropin and human chorionic gonadotropin), was studied. The value of urinary oestrogen measured by this method related closedly to the value of serum oestradiol measured by radioimmunoassay. The micrometering kit was sensitive enough to monitor follicular maturation. It was found that when the level of urinary oestrogen was 75 ng/ml, the chance of ovulation was 75 percent; once the level exceeded 100 ng/ml, all patients had ovulation; when the level was 50 ng/ml or less, there was no ovulation. This method of urinary oestrogen measurement is shown to be useful in clinical practice, because the technique is simple, the results can be obtainted in 2 minutes, and it is sensitive for measuring oestrogen from the level of 2 ng/ml.