Preparation, crystallization and preliminary X-ray crystallographic analysis of OXA-23, a carbapenemase conferring widespread antibiotic resistance.
Indian J Biochem Biophys
;
2011 Dec; 48(6): 395-398
Artículo
en Inglés
| IMSEAR
| ID: sea-140206
ABSTRACT
OXA-23, a class D carbapenemase that confers widespread antibiotic resistance hydrolyzes the β-lactam rings of β-lactam antibiotics, presenting an enormous challenge to infection control, particularly in the eradication of pathogenic bacteria such as Acinetobacter baumannii, one of six top-priority dangerous pathogens. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing carbapenemases. In this study, OXA-23 was purified and crystallized at 298 K and X-ray diffraction data from OXA-23 crystal were collected at 2.03 Å resolution using synchrotron radiation. The crystal of OXA-23 belonged to space group P41 with unit cell parameters a = 82.47, b = 82.47 and c = 172.01 Å. Analysis of the packing density showed that the asymmetric unit probably contained two molecules with a solvent content of 73.64%.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Beta-Lactamasas
/
Farmacorresistencia Microbiana
/
Datos de Secuencia Molecular
/
Secuencia de Aminoácidos
/
Homología de Secuencia de Aminoácido
/
Cristalografía por Rayos X
/
Cristalización
/
Electroforesis en Gel de Poliacrilamida
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2011
Tipo del documento:
Artículo
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