Antifungal susceptibility testing of Candida tropicalis biofilms against fluconazole using calorimetric indicator resazurin.
Indian J Pathol Microbiol
;
2012 Jan-Mar 55(1): 72-74
Artículo
en Inglés
| IMSEAR
| ID: sea-142180
ABSTRACT
Background:
C.tropicalis is an important cause of nosocomial infections particularly in immunocompromised patients. Infections caused by Candida spp. are often associated with biofilm formation on implanted medical devices or on epithelial cell surfaces. Phenotypic characteristics of sessile cells in biofilms are known to be different from those of their free-living, planktonic counterparts. Biofilm forming strains often show increased resistance to antimicrobial agents. Materials andMethods:
We measured susceptibility to fluconazole of fifty C.tropicalis isolates from immunocompromised (29) and immunocompetent (21) patients by minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) assays. MBIC was done using the calorimetric indicator resazurin, to measure the metabolically active cells.Results:
Biofilm forming cells showed increased resistance to fluconazole.Conclusion:
The resazurin dye test was found to be a good method for determining MBIC.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Oxazinas
/
Coloración y Etiquetado
/
Xantenos
/
Humanos
/
Candidiasis
/
Pruebas de Sensibilidad Microbiana
/
Fluconazol
/
Huésped Inmunocomprometido
/
Colorimetría
/
Biopelículas
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
2012
Tipo del documento:
Artículo
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