Your browser doesn't support javascript.
loading
Development of a new method for diagnosis of Group B Coxsackie genome by reverse transcription loop-mediated isothermal amplification.
Indian J Med Microbiol ; 2011 Apr-June; 29(2): 110-117
Artículo en Inglés | IMSEAR | ID: sea-143792
ABSTRACT

Background:

Coxsackie B viruses (genus, Enterovirus; family, Picornaviridae) can cause aseptic meningitis, encephalitis, pleurodynia, and fatal myocarditis, and are implicated in the pathogenesis of dilated cardiomyopathy. The differentiation of the group B Coxsackieviruses into their subtypes has potential clinical and epidemiological implications.

Objective:

In this study, we developed a one-step, single-tube genogroup-specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5′ UTR region. Materials and

Methods:

The amplification can be obtained in less than 1 hour by incubating all the reagents in a single tube with reverse transcriptase and Bst DNA polymerase at 63°C. Detection of gene amplification could be accomplished by agarose gel electrophoresis and the monitoring of gene amplification can also be visualised with the naked eye by using SYBR green I fluorescent dye.

Results:

A total of 40 samples comprising 31 positive samples and 9 negative samples were used in this study for comparative evaluation. The results were compared with those from Real-Time Polymerase Chain Reaction (RT-PCR). None of the RT-PCR-positive samples were missed by RT-LAMP, thereby indicating a higher sensitivity of the RT-LAMP assay.

Conclusion:

Thus, due to easy operation without a requirement of sophisticated equipment and skilled personnel, the RT-LAMP assay reported here is extremely rapid, cost-effective, highly sensitive, and specific and has potential usefulness for rapid detection of non-polio enterovirus (NPEV) not only by well-equipped laboratories but also by peripheral diagnostic laboratories with limited financial resources in developing countries.
Asunto(s)

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Compuestos Orgánicos / Coloración y Etiquetado / Temperatura / Factores de Tiempo / Humanos / ARN Viral / Sensibilidad y Especificidad / Enterovirus Humano B / Técnicas de Laboratorio Clínico / Infecciones por Coxsackievirus Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Indian J Med Microbiol Asunto de la revista: Microbiology Año: 2011 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Compuestos Orgánicos / Coloración y Etiquetado / Temperatura / Factores de Tiempo / Humanos / ARN Viral / Sensibilidad y Especificidad / Enterovirus Humano B / Técnicas de Laboratorio Clínico / Infecciones por Coxsackievirus Tipo de estudio: Estudio diagnóstico Idioma: Inglés Revista: Indian J Med Microbiol Asunto de la revista: Microbiology Año: 2011 Tipo del documento: Artículo