Prevalence and antimicrobial resistance pattern of multidrug-resistant enterococci isolated from clinical specimens.
Indian J Med Microbiol
;
2012 Jan-Mar; 30(1): 44-51
Artículo
en Inglés
| IMSEAR
| ID: sea-143893
ABSTRACT
Purpose:
Vancomycin-resistant enterococci (VRE) pose an emerging problem in hospitals worldwide. The present study was undertaken to determine the occurrence, species prevalence, antibacterial resistance, and phenotypic and genetic characteristics of VRE isolated in Riyadh hospitals, KSA. Materials andMethods:
Two hundred and six isolates of enterococcal species were obtained from clinical samples. The antibiotic susceptibility of isolates and minimum inhibitory concentration (MIC) tests for vancomycin and teicoplanin were determined. Molecular typing of VRE isolates was carried out by using pulsed field gel electrophoresis (PFGE) and the resistance genotype was determined by polymerase chain reaction (PCR).Results:
VRE accounted for 3.9% of the isolates and were detected mostly in urine, wound and blood specimens isolated from ICU, internal medicine and surgical wards. All strains were identified to species level and were found to consist of E. faecalis (69.2%), E. faecium (11.3%), E. avium (2.1%), E. hirae (0.8%), E. casseliflavus (1.3%) and E. gallinarum (1.3%) species. According to the susceptibility data obtained, 8 (3.9%) out of 206 isolates were found to be VRE (MICs > 32 μg/ml). The vanA, vanB and vanC gene fragments of E. faecalis, E. faecium and E. gallinarum were amplified from isolates and were detected. PFGE patterns of the VRE isolates revealed homogenous patterns with dominant clone suggesting that the strains intrinsic resistance is independent.Conclusions:
This study shows an emergence of VRE along with increased rate of multidrug-resistant enterococci in the area of the study. Regular surveillance of antimicrobial susceptibilities should be done regularly and the risk factors should be determined.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Arabia Saudita
/
Humanos
/
Pruebas de Sensibilidad Microbiana
/
Reacción en Cadena de la Polimerasa
/
Prevalencia
/
Infecciones por Bacterias Grampositivas
/
Enterococcus
/
Electroforesis en Gel de Campo Pulsado
/
Farmacorresistencia Bacteriana Múltiple
/
Tipificación Molecular
Tipo de estudio:
Estudio de prevalencia
/
Estudio pronóstico
/
Factores de riesgo
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Indian J Med Microbiol
Asunto de la revista:
Microbiology
Año:
2012
Tipo del documento:
Artículo
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