First characterisation of plasmid-mediated quinolone resistance-qnrS1 co-expressed bla CTX-M-15 and bla DHA-1 genes in clinical strain of Morganella morganii recovered from a Tunisian Intensive Care Unit.
Indian J Med Microbiol
;
2012 Oct-Dec; 30(4): 437-441
Artículo
en Inglés
| IMSEAR
| ID: sea-144006
ABSTRACT
Purpose:
Aim of this study was to show the emergence of the qnr genes among fluoroquinolone-resistant, AMPC and ESBL (extended-spectrum-beta-lactamase) co-producing Morganella morganii isolate. Materials andMethods:
A multi resistant Morganella morganii SM12012 isolate was recovered from pus from a patient hospitalized in the intensive care unit at the Military hospital, Tunisia. Antibiotic susceptibility was tested with the agar disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. ESBLs were detected using a standard double-disk synergy test. The characterization of beta-lactamases and associated resistance genes were performed by isoelectric focusing, polymerase chain reaction and nucleotide sequencing.Results:
The antimicrobial susceptibility testing showed the high resistance to penicillins, cephalosporins (MICs 64-512 μg/ml) and fluoroquinolones (MICs 32-512 μg/ml). But M. morganii SM12012 isolate remained susceptible to carbapenems (MICs 4-<0.25 μg/ml). The double-disk synergy test confirmed the phenotype of extended-spectrum β-lactamases (ESBLs). Three identical β-lactamases with pI values of 6.5, 7.8 and superior to 8.6 were detected after isoelectric focusing analysis. These β-lactamases genes can be successfully transferred by the conjugative plasmid. Molecular analysis demonstrated the co-production of bla DHA-1, bla CTX-M-15 and qnrS1 genes on the same plasmid. The detection of an associated chromosomal quinolone resistance revealed the presence of a parC mutation at codon 80 (Ser80-lle80).Conclusion:
This is the first report in Tunisia of nosocomial infection due to the production of CTX-M-15 and DHA-1 β-lactamases in M. morganii isolate with the association of quinolone plasmid resistance. The incidence of these strains invites continuous monitoring of such multidrug-resistant strains and the further study of their epidemiologic evolution.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Plásmidos
/
Proteínas Bacterianas
/
Túnez
/
Beta-Lactamasas
/
Humanos
/
Masculino
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Infección Hospitalaria
/
Quinolonas
Tipo de estudio:
Guía de Práctica Clínica
País/Región como asunto:
Africa
Idioma:
Inglés
Revista:
Indian J Med Microbiol
Asunto de la revista:
Microbiology
Año:
2012
Tipo del documento:
Artículo
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