Rapid detection of chromosome X, Y, 13, 18, and 21 aneuploidies by primed in situ labeling/synthesis technique.
Indian J Hum Genet
;
2013 Jan; 19(1): 14-17
Artículo
en Inglés
| IMSEAR
| ID: sea-147631
ABSTRACT
AIMS AND OBJECTIVE:
Primed in situ labeling/synthesis (PRINS) technique is an alternative to fluorescent in situ hybridization for chromosome analysis. This study was designed to evaluate the application of PRINS for rapid diagnosis of common chromosomal aneuploidy. MATERIALS ANDMETHODS:
We have carried out PRINS using centromere specific oligonucleotide primers for chromosome X, Y, 13, 18 and 21 on lymphocyte metaphase and interphase cells spread. Specific primer was annealed in situ, followed by elongation of primer by Taq DNA polymerase in presence of labeled nucleotides. Finally, reaction was stopped and visualized directly under fluorescent microscope.RESULTS:
Discrete centromere specific signals were observed with each primer.CONCLUSION:
PRINS seems to be a rapid and reliable method to detect common chromosome aneuploidy in peripheral blood lymphocyte metaphase and interphase cells.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Cromosoma X
/
Cromosoma Y
/
Cromosomas Humanos Par 13
/
Cromosomas Humanos Par 18
/
Cromosomas Humanos Par 21
/
Humanos
/
Cromosomas Humanos
/
Etiquetado in Situ Primed
/
Aneuploidia
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Hum Genet
Año:
2013
Tipo del documento:
Artículo
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