Optimization of Nutritive Factors in Culture Media for Growth and Tropane Alkaloid Production from Anisodus acutangulus Hairy Roots.
Artículo
en Inglés
| IMSEAR
| ID: sea-151498
ABSTRACT
Anisodus acutangulus hairy roots were grown in N6 medium which was optimal for growth and alkaloid production. The cell biomass and alkaloid yield reached 2.7 g l-1 and 3.9 mg l-1 (dry weight) respectively in sucrose medium, higher than those obtained in other carbon source media. 90 mM nitrogen (NH4 +/NO3 – = 41) gave the highest cell yield (4.5 g l-1) and the maximum alkaloid production (9.9 mg l-1). The cell yield (4.1 g l-1) of hairy roots grown at pH 6.5 was 2 times higher than that at pH 4.5. However, the maximum alkaloid production (7.2 mg l-1) was yielded at pH 4.5.
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Índice:
IMSEAR (Asia Sudoriental)
Idioma:
Inglés
Año:
2013
Tipo del documento:
Artículo
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