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Biological activity and redistribution of nucleolar proteins of two different cell lines treated with cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP).
Indian J Exp Biol ; 2014 Jun; 52(6): 579-588
Artículo en Inglés | IMSEAR | ID: sea-153736
ABSTRACT
The interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and fibrillarin) involved in DNA transcription, rRNA processing, and ribosomal assembly. The results showed that RAP binds to the DNA of two cell lines (H4 and Hs-683) causing a delay in cell proliferation rate leading to a number of cellular modifications. These modifications include DNA-damage assessed by the single cell gel electrophoresis method (comet assay) and variation in the expression of nucleolar proteins; UBF was more abundant in RAP treated cells, this was explained by the high affinity of this protein to DNA modified by RAP. On the other hand, fibrillarin was found in less quantities in RAP treated cells which was explained by a de-regulation of the ribosomal machinery caused by RAP.
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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Compuestos Organometálicos / Transcripción Genética / Daño del ADN / Humanos / Proteínas Nucleares / Distribución Tisular / Procesamiento Postranscripcional del ARN / Transporte de Proteínas / Línea Celular Tumoral / Evaluación Preclínica de Medicamentos Idioma: Inglés Revista: Indian J Exp Biol Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Compuestos Organometálicos / Transcripción Genética / Daño del ADN / Humanos / Proteínas Nucleares / Distribución Tisular / Procesamiento Postranscripcional del ARN / Transporte de Proteínas / Línea Celular Tumoral / Evaluación Preclínica de Medicamentos Idioma: Inglés Revista: Indian J Exp Biol Año: 2014 Tipo del documento: Artículo