Development of bioprocess for the production of laccase by Pleurotus ostreatus MTCC 1802 using evolutionary optimization technique.
Indian J Exp Biol
;
2014 Nov; 52(11): 1106-1111
Artículo
en Inglés
| IMSEAR
| ID: sea-153798
ABSTRACT
For cost effective production of laccase enzyme (benzenediol oxygen oxidoreductase) from P. ostreatus MTCC 1802 through solid sate fermentation, physico-chemical parameters such as temperature (20-35 ºC), incubation period (9-17 days) and substrate (Neem bark and wheat bran, in various ratios, w/w) were optimized first by one parameter at time approach and then obtained optimum conditions were considered as zero level in evolutionary optimization factorial design technique. At statistically optimized conditions yield of laccase was found 303.59+16.8) U/gds after 13 days of incubation at 25 ºC taking wheat bran and neem bark as substrate at a ratio of 32 (w/w). The results obtained could be a base line for industrial scale production of laccase.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Oryza
/
Temperatura
/
Proteínas Fúngicas
/
Fibras de la Dieta
/
Tallos de la Planta
/
Pleurotus
/
Medios de Cultivo
/
Corteza de la Planta
/
Toma de Decisiones
/
Azadirachta
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2014
Tipo del documento:
Artículo
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