Analysis on the arcelin expression in bruchid pest resistant wild pulses using real time RT-qPCR.
Indian J Exp Biol
;
2014 Dec; 52(12): 1195-1200
Artículo
en Inglés
| IMSEAR
| ID: sea-153811
ABSTRACT
Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gene specific real time PCR primers were synthesized from arcelin mRNA sequence of the wild pulse variety, Lablab purpureus. The results revealed different levels of arcelin gene expression in the tested varieties. Canavalia virosa registered significantly high content indicating its suitability for utilization of arcelin gene in developing stored product insect pest resistance with other cultivated pulses.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Enfermedades de las Plantas
/
Semillas
/
Especificidad de la Especie
/
Escarabajos
/
Glicoproteínas
/
Regulación de la Expresión Génica de las Plantas
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Phaseolus
/
Canavalia
/
Resistencia a la Enfermedad
Idioma:
Inglés
Revista:
Indian J Exp Biol
Año:
2014
Tipo del documento:
Artículo
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