Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.
Indian J Pathol Microbiol
;
2013 Jul-Sept 56 (3): 243-247
Artículo
en Inglés
| IMSEAR
| ID: sea-155876
ABSTRACT
Background:
The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion.Aim:
In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials andMethods:
Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 11200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL.Results:
Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Indian J Pathol Microbiol
Año:
2013
Tipo del documento:
Artículo
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