A Comparative Study of Laboratory Diagnosis ofGenital Chlamydial Infections InWomen By Immunofluorescence And By Conventional Staining Method.

ABSTRACT

Objective: The major objective of this study was to carry out concurrent testing of cervical swab samples with both conventional and fluorescent staining method using Giemsa stain & direct fluorescent antibody stain (DFA) respectively. The study would enable us to establish an appropriate, effective and sensitive method of diagnosis of genital chlamydial infections within the present set-up.Material & methods: The study “A Comparative Study of laboratory Diagnosis of Genital Chlamydial Infections in Women by Immunofluorescence and Conventional Staining Method” was conducted on 50 patients attending the OPD of a tertiary care hospital of Ahmedabad. Results: Out of the total 50 cases tested, four samples were found positive containing dark-purple inclusion bodies of Chlamydia trachomatis surrounding the nuclei of the host cells in Giemsa and the same were positive for elementary bodies of C. trachomatis by the DFA staining. Based on these samples, the prevalence of is 8% only.Conclusion: The present comparative study of Giemsa and DFA staining for Chlamydia trachomatis infections in females showed that both methods are equally sensitive for the detection of the microorganism.