Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.
Indian J Biochem Biophys
;
2015 Feb; 52 (1): 107-110
Artículo
en Inglés
| IMSEAR
| ID: sea-157962
ABSTRACT
Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Espectrometría de Fluorescencia
/
Anticuerpos Antinucleares
/
Técnica del Anticuerpo Fluorescente
/
Fotoblanqueo
/
Pulmón
Idioma:
Inglés
Revista:
Indian J Biochem Biophys
Año:
2015
Tipo del documento:
Artículo
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