Studies On ApoB VNTR In Two Maharashtrian Ethnic population Groups.

ABSTRACT

The ApoB VNTR locus was analyzed in two distinct ethnic groups of Maharashtra to determine, the distribution of allele and genotype frequencies. Blood samples were collected from 183 random, unrelated, healthy donors. DNA was extracted by using a simple salt precipitation method, amplified by polymerase chain reaction (PCR) and the products were electrophoresed in 4% PAGE followed by silver staining. A total of 14 alleles and 38 genotypes were observed. Allele 37 and 39 were found to be the predominant alleles showing a bimodal distribution. Both the population groups conformed to Hardy-Weinberg Equilibrium expectations.