Animal model for testing human Ascaris allergens.

ABSTRACT

Guinea pigs immunized through the nasal route by an antigenic preparation of human Ascaris lumbricoides produced predominantly homocytotropic antibodies. The sensitization of the homologous skin required a latency period. Hypersensitivity reaction was triggered within 30 min. of the antigen challenge. The antibodies were sensitive to heat and β–mercaptoethanol treatments and appeared to be similar to the IgE type of immunoglobulins of man and rabbit. The antigenic preparation elicited an immediate type of skin hypersensitivity reaction also in human subjects harbouring the Ascaris parasite. The guinea pig model appears suitable for testing the activity of Ascaris allergens.