Towards a subunit influenza vaccine prepared by affinity chromatography on immobilized lectin.
J Biosci
;
1983 Dec; 5(suppl_1): s149-s155
Artículo
en Inglés
| IMSEAR
| ID: sea-160292
ABSTRACT
Disintegration of influenza virions with 0.2% w/v sodium deoxycholate releases haemagglutinin and neuraminidase, which in the presence of detergent are both adsorbed to the lectin from Crotalaria juncea, coupled to Sepharose 2B. The binding is mediated through galactosyl residues on haemagglutinin and neuralminidase, which are completely displaced in 0.2 Μ lactose and co-elute in a narrow zone. The immunogenicity of haemagglutinin and neuraminidase in mice is markedly increased after adsorption onto lipid, particles constituting “intralipid” (Kabi Vitrum, Stocholm, Sweden).
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Idioma:
Inglés
Revista:
J Biosci
Año:
1983
Tipo del documento:
Artículo
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