Your browser doesn't support javascript.
loading
Heat shock response of Chlorella protothecoides during greening.
J Biosci ; 1987 Sept; 12(3): 219-228
Artículo en Inglés | IMSEAR | ID: sea-160582
ABSTRACT
Heterotrophically grown cells of Chlorella protothecoides were transferred to autotrophic medium and allowed to green at 25°C. The protein synthetic activity of the greening cells measured in terms of incorporation of [35S]-methionine showed a maximum around 20 h of greening and thereafter started declining. Similarly, an analysis of densitometric tracings of the fluorographic profile of the polypeptides associated with both total cellular fraction and membrane fractions during different hours of greening revealed that maximum number of polypeptides were getting labelled around 20 h of greening. At 20 h of greening, the cells were shifted to 40°C and the effect of heat shock on protein synthesis was studied. The heat shock treatment caused a definite decrease in the incorporation of [35S]- methionine into proteins. Due to heat shock, the synthesis of total soluble proteins was affected much more than that of the thylakoid membrane bound proteins. When the cells were transferred back to 25°C after a brief period of heat shock at 40°C, there was a considerable recovery in the protein synthesis and this recovery was found to be significant in the case of soluble proteins, while there was no such definite recovery in the synthesis of thylakoid membrane bound proteins.

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Idioma: Inglés Revista: J Biosci Año: 1987 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Idioma: Inglés Revista: J Biosci Año: 1987 Tipo del documento: Artículo