Prostaglandin-synthetase activity in developing toad ovary— I. Detection and properties.
J Biosci
;
1986 Mar; 10(1): 75-83
Artículo
en Inglés
| IMSEAR
| ID: sea-160592
ABSTRACT
Prostaglandin-synthetase activity has been measured in the microsomal fraction of developing toad (Bufo melanostictus) ovary using arachidonic acid as the substrate. Indomethacin (0·74 μΜ) and aspirin (0·35 μΜ) inhibit this activity. The activity is maximum in immature ovary and its level gradually decreases with maturity of the organ till the breeding season arrives, when it rises again. Time course study shows that the activity in vitro becomes steady after 3 min of incubation in all the cases, except the immature ones in which it sharply declines. Soluble supernatant was found to contain some inhibitory factor(s), which is partially inactivated by heating at 100°C for 5 min (~ 43%). Intraperitoneal injection of equine luteinizing hormone stimulates this enzyme activity in the mature ovary during non-breeding season. This suggests that similar to mammalians prostaglandin-synthetase, the toad ovary enzyme is also regulated by luteinizing hormone.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
J Biosci
Año:
1986
Tipo del documento:
Artículo
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