Ethanol extract of rosemary repressed pten expression in K562 culture cells.

ABSTRACT

Several medicinal herbs are found to have a potent transcriptional activity for the gene involved in cellular function. In the present study, we aimed to determine the effects of such herbs on PTEN (phosphatase and tensin homolog deleted on chromosome 10) gene, which is a candidate gene involved in cancer development. The in vitro effect of a Rosemary (Rosmarinus officinalis) extract on the reduction of PTEN expression was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, western blotting also confirmed the down-regulation of the protein at dose dependent manner of the Rosemary extract. Our results show that component(s) of Rosemary inhibits the expression of PTEN in K562 myeloid cell line cells.