Cytokine generation in stored platelet concentrate: comparison of two methods of preparation.
Artículo
en Inglés
| IMSEAR
| ID: sea-16224
ABSTRACT
BACKGROUND & OBJECTIVES:
Contaminating white blood cells (WBCs) in stored platelet concentrates (PC) are the main source of pro-inflammatory cytokines including interleukin-6 (IL-6), interleukin- 8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) that are implicated in transfusion reactions. We compared the levels of these cytokines in stored platelet preparations prepared by two methods. Effect of pre-storage leucofiltration on these cytokine levels was also studied.METHODS:
Twelve units of pooled PCs were prepared by platelet rich plasma (PRP) method and buffy-coat (BC) method each and stored for 5 days. IL-6, IL-8 and TNF-alpha levels were measured in platelet supernatants on day 0, 1, 3 and 5 of the storage using commercially available immunoassays. Pre-storage leucofiltration was done in 4-pooled units of PRP-PC and cytokine levels compared with unfiltered PCs.RESULTS:
Median IL-6 levels increased from day 0 to day 5 in both PRP-PC and BC-PC. In PRP-PC, IL-8 increased from <3 pg/ml on day 0 to 817 pg/ml on day 5, while in BC-PC the corresponding levels were 10 and 346.5 pg/ml, respectively. No significant increase in levels of TNF-alpha was observed in BC-PC during storage period, while levels increased significantly in PRP-PC on day 1 only. There was no significant change in the levels of all three cytokines in leucofiltered PCs over 5 days of storage. INTERPRETATION &CONCLUSION:
Findings of our study showed that method of preparation and WBC content are the critical factors in determining the cytokine levels in stored PCs.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Factores de Tiempo
/
Plaquetas
/
Conservación de la Sangre
/
Humanos
/
Separación Celular
/
Estudios Prospectivos
/
Citocinas
/
Interleucina-8
/
Interleucina-6
/
Factor de Necrosis Tumoral alfa
Tipo de estudio:
Estudio observacional
Idioma:
Inglés
Año:
2006
Tipo del documento:
Artículo
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