Quantitative Estimation of Piperine and Diosgenin in Ayurvedic Formulation.

ABSTRACT

Goksuradi guggulu is a polyherbal formulation official in “Ayurvedic formulary of India” and used for dysuria, urinary obstruction, increased frequency and turbidity of urine, calculus, excessive vaginal discharge, gout. A simple, specific and precise high performance thinlayer chromatography (HPTLC) method has been developed for quantification of piperine and diosgenin in Goksuradi guggulu. We report the extraction and estimation of these compounds in a laboratory prepared sample of Goksuradi guggulu and two of its marketed formulations. The compounds were chromatographed on precoated silica gel G 60 254 plates in the mobile phase comprising of toluene hexane ethyl acetate (6.80.23). Under the optimized chromatographic conditions, the calibration plot was found to be linear in the range of 0.2-1μg spot-1 for piperine and 1.0 -3.0 μg spot-1 for diosgenin. The correlation coefficient (R2) was 0.9979 and 0.9915 for piperine and diosgenin respectively. Mean recovery (% w/w) for piperine in all the formulations was in the range of 97.61 - 98.90 and for diosgenin, it was 93.76 - 94.33.