Application of Liquid Chromatography-Mass Spectrometry for Detecting Terlipressin Levels in Rat Plasma.

ABSTRACT

This work describes a liquid chromatography– electrospray tandem mass spectrometry method for detection of terlipressin in Wistar rat plasma in the low nano-gram range. Terlipressin is a synthetic analogue of the antidiuretic hormone arginine vasopressin and it might be used when Arginine vasopressin (AVP) is not readily available. For experimental evaluation of the pharmacokinetics of terlipressin, rats can be injected 3.0 μg/kg or 6.0 μg/kg i.v. of terlipressin and blood can be collected for Mass spectrometry characterization of terlipressin which can be performed with a high-resolution Orbitrap-based mass spectrometer.