Screening of in-vitro antioxidant activity of Hippophae salicifolia berries extract.

ABSTRACT

The present study aimed to study the preliminary phytochemical analysis, standardisation and in vitro antioxidant evaluation of Hippophae salicifolia (HS) berries extracts. Briefly, the berries were subjected to alcoholic and aqueous extraction. The preliminary phytochemical analysis was performed for aqueous and alcoholic extracts. The aqueous and alcoholic extracts are standardised for total phenolic, total tannin and total flavonoid content. The extracts were evaluated for its in-vitro free radical scavenging activities against, 2’-diphenyl-1- picrylhydrazyl (DPPH) radicals, hydroxyl radicals, nitric oxide radicals and total antioxidant activity. We found that alcoholic extract showed the presence of alkaloids, phenols,tannins, saponins, flavonoids and aqueous extract showed the presence of flavonoids, tannins, carbohydrates, and phenols. The extracts of HS berries were standardized for their total phenolic, tannin, flavonoid and vitamin-C content. The in vitro antioxidant activity revealed that both the extracts showed significant scavenging activities against free radicals. It can be concluded that both the extracts are effective in scavenging the free radicals. The flavonoids, tannins, vitamin-c and saponins may be attributed to free radical quenching activity of Hippophae salicifolia.