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Development, characterization & potential clinical use of monoclonal antibodies against human alphafoetoprotein.
Artículo en Inglés | IMSEAR | ID: sea-16630
ABSTRACT
Four anti-alphafoetoprotein (AFP) monoclonal antibodies (MAb) were raised in the laboratory and characterized using ELISA and immunodot assays. The affinity constants of the MAbs, analysed by scatchard plots, ranged from 3.1 X 10(8) to 2.15 X 10(9) M/l. Epitope analysis using competition RIA indicated that MAb 5E2D7 and 5E2E3 recognize different epitopes on AFP. This combination was used to set up a two site sandwich ELISA with HRPO conjugated 5E2D7. AFP values in sera of hepatocellular carcinoma patients and pregnant women were quantitated using sandwich ELISA. The anti-AFP MAbs showed strong reactivity when tested on hepatoma tissue sections using immunoperoxidase technique.
Asunto(s)
Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Humanos / Embarazo / Ensayo de Inmunoadsorción Enzimática / Immunoblotting / Alfa-Fetoproteínas / Carcinoma Hepatocelular / Neoplasias Hepáticas / Anticuerpos Monoclonales Idioma: Inglés Año: 1989 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Asunto principal: Femenino / Humanos / Embarazo / Ensayo de Inmunoadsorción Enzimática / Immunoblotting / Alfa-Fetoproteínas / Carcinoma Hepatocelular / Neoplasias Hepáticas / Anticuerpos Monoclonales Idioma: Inglés Año: 1989 Tipo del documento: Artículo