Development, characterization & potential clinical use of monoclonal antibodies against human alphafoetoprotein.
Artículo
en Inglés
| IMSEAR
| ID: sea-16630
ABSTRACT
Four anti-alphafoetoprotein (AFP) monoclonal antibodies (MAb) were raised in the laboratory and characterized using ELISA and immunodot assays. The affinity constants of the MAbs, analysed by scatchard plots, ranged from 3.1 X 10(8) to 2.15 X 10(9) M/l. Epitope analysis using competition RIA indicated that MAb 5E2D7 and 5E2E3 recognize different epitopes on AFP. This combination was used to set up a two site sandwich ELISA with HRPO conjugated 5E2D7. AFP values in sera of hepatocellular carcinoma patients and pregnant women were quantitated using sandwich ELISA. The anti-AFP MAbs showed strong reactivity when tested on hepatoma tissue sections using immunoperoxidase technique.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Femenino
/
Humanos
/
Embarazo
/
Ensayo de Inmunoadsorción Enzimática
/
Immunoblotting
/
Alfa-Fetoproteínas
/
Carcinoma Hepatocelular
/
Neoplasias Hepáticas
/
Anticuerpos Monoclonales
Idioma:
Inglés
Año:
1989
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS