Active Case Detection and Prevalence of Urinary Schistosomiasis and Malaria in Pupils of Kotto Barombi, Southwest Cameroon Using the CyScope® Fluorescence Microscope.
Artículo
en Inglés
| IMSEAR
| ID: sea-166984
ABSTRACT
Aim:
This study was aimed at assessing the use of the CyScope® fluorescence microscope to determine the prevalence of urinary schistosomiasis (US) and malaria in Kotto Barombi. ExperimentalDesign:
The study was a cross-sectional survey. Place and Duration of Study The study was carried out in Kotto Barombi, Cameroon from April to May, 2013.Methodology:
Urine and blood samples were collected from 216 pupils. US eggs were detected in urine by centrifugation and CyScope® methods for schistosome eggs. Malaria parasites were detected using Giemsa-stained blood films and CyScope® methods. The performance characteristics of the CyScope® for both infections were determined using light microscopy as gold standard.Results:
Overall prevalence of US was 43.4% and 48.5% by light microscopy and CyScope® respectively. Prevalence of US was significantly higher (P<0.01) in the Kotto Barombi Island (78.3%) than Mainland (33.8%). US prevalence was not affected by age, sex and socio-economic class (SEC). Mean intensity of US was 8.1 eggs/10 ml urine (Confidence interval, CI = 4.3–11.9). It was significantly higher (P =.01) in pupils from Island (36.5 eggs/10 ml, CI 17.7–55.3) than Mainland (8.8 eggs/10 ml; 7.1–10.5), males (19.2 eggs/10 ml urine; CI 9.2–29.2) than females (17.8 eggs/10 ml urine; CI 13.1–22.5) and highest (P = .046) in the ≤6 years age group (36.9 eggs/10 ml; CI 20.4–53.4) when compared with pupils in other age groups. Sensitivity and specificity of CyScope® for US were 90.6% and 83.8% respectively. Overall prevalence of malaria was 19.0% and 41.2% by light microscopy and CyScope® respectively and the difference was significant (P = .01). Malaria prevalence and density were not influenced by age; sex and SEC. Sensitivity and specificity of CyScope® for malaria were 68.3% and 64.9% respectively.Conclusion:
The CyScope® could be a useful tool for active case detection of both diseases especially in areas that lack electricity.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de prevalencia
/
Factores de riesgo
Idioma:
Inglés
Año:
2015
Tipo del documento:
Artículo
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