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How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India.
Indian J Med Microbiol ; 2015 Oct-Dec; 33(4): 482-490
Artículo en Inglés | IMSEAR | ID: sea-176501
ABSTRACT
Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development.

Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Idioma: Inglés Revista: Indian J Med Microbiol Asunto de la revista: Microbiology Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: IMSEAR (Asia Sudoriental) Idioma: Inglés Revista: Indian J Med Microbiol Asunto de la revista: Microbiology Año: 2015 Tipo del documento: Artículo