Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene.
Artículo
en Inglés
| IMSEAR
| ID: sea-17776
ABSTRACT
BACKGROUND & OBJECTIVE:
We report a new polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples.METHODS:
The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium-M. intracellulare complex (MAC) clinical isolates (Of which 31 were from HIV(+) individuals). The standard and clinical strains were typed with HPLC based mycolic acid fingerprinting.RESULTS:
Three polymorphisms (BamHI, BstNI and HgaI) were identified for inter-species differentiation among standard strains; of which, only HgaI was found to be useful in clinical isolates. Of the 45 isolates, 25 were M. avium and 20 were M. intracelluare. MAC isolates, which could not be differentiated by HPLC analysis, were also typed by this method. INTERPRETATION &CONCLUSION:
The use of mycobacterial groES as a PCR-RFLP target for M. avium and M. intracellulare is a simple and rapid method that can complement HPLC in their differentiation.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Especificidad de la Especie
/
Proteínas Bacterianas
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Humanos
/
ADN Bacteriano
/
Secuencia de Bases
/
Complejo Mycobacterium avium
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Chaperonina 10
Idioma:
Inglés
Año:
2007
Tipo del documento:
Artículo
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