Production & evaluation of a monoclonal antibody against human myeloperoxidase.
Artículo
en Inglés
| IMSEAR
| ID: sea-17923
ABSTRACT
We describe the production of a mouse monoclonal antibody (H2E1) against human myeloperoxidase antigen. After production and characterisation, this antibody was compared with commercially available monoclonal antibodies, cytochemical myeloperoxidase and previously produced polyclonal antibody. Reaction with various cell lines proved that this monoclonal antibody was specific for myeloid lineage. This monoclonal showed positivity in 81.8 per cent of acute myeloid leukaemias whereas the polyclonal antibody was 100 per cent positive. We found that the polyclonal antibody was more sensitive as compared to the monoclonal. This is probably due to the lack of recognition of individual epitopes on the antigen. We recommend the use of antibodies which have different epitope recognition as most specific for myeloperoxidase.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Humanos
/
Inmunohistoquímica
/
Células Tumorales Cultivadas
/
Leucemia Mieloide
/
Inmunofenotipificación
/
Peroxidasa
/
Estudios de Evaluación como Asunto
/
Animales
/
Ratones
/
Anticuerpos Monoclonales
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
Idioma:
Inglés
Año:
1997
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS