Evaluate “Rifampicin Resistance” as Surrogate Marker for Rapid Detection of MDR-TB Using Real-Time PCR Directly on FNAC Samples of Tuberculous Lymphadenitis.
Br J Med Med Res
;
2015; 9(5): 1-8
Artículo
en Inglés
| IMSEAR
| ID: sea-180955
ABSTRACT
Background:
India has the dubious distinction of having second largest burden of MDR-TB cases in the world. According to WHO, MDR-TB is defined as resistance to isoniazid and rifampicin, the two most important drugs for treatment of TB. “Rifampicin resistance” is recommended as surrogate marker for MDR-TB by WHO, as at least, 90% of all rifampicin-resistant clinical isolates are also found resistant to isoniazid. Localization of genetic alterations in the 81-bp “Rifampicin Resistance-Determining Region” of rpoB gene in 96% of rifampicin resistant strains make it particularly amenable for early detection of MDR-TB by molecular techniques like Real-Time PCR.Aim:
Evaluation of “rifamipicin resistance” as surrogate marker for rapid detection of MDR-TB using Real-Time PCR directly on FNAC samples of tuberculous lymphadenitis (TBLN). Materials andMethods:
Eighty cases of TBLN undergoing anti-tubercular treatment (ATT) and 10 lymphadenitis cases of non-tuberculous origin (controls) were included in the study. To evaluate “rifamipicin resistance” as surrogate marker for rapid detection of MDR-TB, Real-Time PCR and conventional Drug Susceptible Testing (DST) were carried out.Results:
Eighteen samples were identified as MDR-TB cases by DST. Real-Time PCR picked up mutated ropB gene in 17 cases out of these 18 MDR-TB cases.Conclusion:
“Rifampicin resistance” is an efficient surrogate marker for timely detection of MDR-TB using rapid, accurate and sensitive molecular technique like Real-Time PCR.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Br J Med Med Res
Año:
2015
Tipo del documento:
Artículo
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