Antifungal Action of the Dillapiole-rich Oil of Piper aduncum against Dermatomycoses Caused by Filamentous Fungi.

ABSTRACT

Aims and Study Design: Piper aduncum L. is a Brazilian plant with many biological properties attributed to its dillapiole-rich essential oil. Despite the development of antibiotics, bacterial and fungal infections are still a public health issue in the medical field. This study measured the antimicrobial activity of the dillapiole-rich essential oil of P. aduncum against pathogenic skin microorganisms. Place of Study Faculty of Pharmacy and Graduate Program in Pharmaceutical Science, Federal University of Pará, Brazil. This work was performed in 2014. Methodology: Gas Chromatography (GC) and Gas Cromatography-Mass spectrometry (GC-MS) have analyzed the oil and its dillapiole-rich fraction. The determination of Minimum Inhibitory Concentration (MIC) and Minimum fungicidal concentration (MFC) values was carried out by microdilution method and counting of formed colonies. Results: For the strains of Trichophyton mentagrophytes (ATCC 9533 and clinical isolate), the oil and its dillapiole-rich fraction exhibited MIC values of 500 μg/ml while the MFC values were 1,500 μg/ml for the oil and 1,000 μg/ml for the fraction rich in dillapiole. For clinical isolates of T. rubrum and Epidermophyton floccosum, MIC values of 500 μg/ml and MFC 1,500 μg/ml were equal for the oil and the dillapiole-rich fraction, respectively. For clinical isolates of Microsporum canis and M. gypseum, the MIC and MFC values were 250 μg/ml and 500 μg/ml, respectively. For strains of Aspergillus fumigatus (ATCC 40152 and clinical isolate), the oil and its dillapiole-rich fraction have shown the same MIC value of 3.9 μg/ml while the MFC values were 7.8 μg/ml for the strain ATCC 40152, and 15.6 μg/ml for the clinical isolate. The oil and dillapiole-rich fraction did not show antibacterial activity against the strain of Staphylococcus aureus ATCC 6538 and its clinical isolate Conclusion: The dillapiole-rich essential oil of P. aduncum and its dillapiole-rich fraction demonstrates significant antifungal activity against dermatophytes, filamentous fungi and potent antifungal activity against non-dermatophyte filamentous fungi.