Neuroprotection Effects of Celastrus paniculatus Seed Oil against Monosodium Glutamate in Human IMR-32 Cells

ABSTRACT

Aims: The aim of this study is to evaluate the neuroprotective effects of Celastrus paniculatus seed oil (CPO) against monosodium glutamate in human IMR-32 cells. Study Design: Celastrus paniculatus seed oil used historically in Indian subcontinent for its neuro-enhancement property and also considered to have free radical scavenging activity. Methodology: In present study we have employed IMR-32, a neuroblastoma cells as our model system and utilized monosodium glutamate (MSG) a widely used food additive and proven inducer of free radicals to study the ameliorative effect of CPO against induced oxidative stress in neuronal cells. Results: Results showed that CPO ameliorates total protein level, decreases protein carbonyl and lipid peroxidation levels (p<0.001) as well as enhances the activity of superoxide dismutase and catalase (p<0.001) under oxidative stress conditions. Further we found that CPO increases the free radical scavenging capacity of cell by enhancing (p<0.001) glutathione level and help its regeneration by revitalizing the activity of glutathione peroxidase, glutathione S-transferase and glutathione reductase enzymes. Conclusion: It can be concluded that CPO has antioxidant property and proved to have ameliorative role against free radicals induced neuronal impairment.