Detection of haemoglobin variants for the diagnosis of beta thalassaemia & other haemoglobinopathies using anion exchange high performance liquid chromatography.
Artículo
en Inglés
| IMSEAR
| ID: sea-18996
ABSTRACT
We describe the precise identification, separation and quantitation of several haemoglobin (Hb) variants such as Hbs A2, A, F, S, E, J, H, D-Punjab and Q-India in untransfused patients with beta thalassaemia and other haemoglobinopathies prevalent in the Indian population using whole blood (5 microliters), red cell lysates (2 microliters) or dried blood specimens on filter paper, using a conventional anion exchange high performance liquid chromatography (AX-HPLC) system. Samples were simultaneously studied for the presence of haemoglobin variants by alkaline electrophoresis. The problems in analysing rare variants and separating the closely migrating variants, encountered in the conventional methods could be resolved using the HPLC technique.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Variación Genética
/
Femenino
/
Humanos
/
Masculino
/
Hemoglobinas
/
Estudios de Casos y Controles
/
Niño
/
Cromatografía Líquida de Alta Presión
/
Cromatografía por Intercambio Iónico
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Talasemia beta
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio pronóstico
Idioma:
Inglés
Año:
1996
Tipo del documento:
Artículo
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