Rapid in vitro adventitious rooting and proliferation by leaf and nodal cultures of Momordica cymbalaria Fenzl.

ABSTRACT

An effective approach for rapid in vitro rooting and proliferation of leaf and nodal cultures of Momordicacymbalaria has been developed. To the ability of induction of rhizogenesis, both leaf and nodal explants wereused in culture on Murashige and Skoog (MS) medium. The effects of auxins such as α-naphthaleneaceticacid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) at different concentrations have beenstudied. The maximum number of roots was produced from nodal explants containing 1.5 mg/L of NAA (9.3 ±0.61), 1.0 mg/L of IBA (6.5 ± 0.41), and 1.0 mg/L of IAA (3.5 ± 0.66), and in leaf explants containing 1.0 mg/Lof NAA (5.7 ± 0.56), 1.0 mg/L of IBA (6.9 ± 0.61), and 1.5 mg/L of IAA (5.0 ± 0.73) on the half-strength MSmedium. For the root induction, NAA is the very effective auxin in node explants of M. cymbalaria. Moreover,a large amount of quercetin bioactive compound is presented in the roots, which is used in anticancer drugs, andwe have described an effective method for the in vitro rhizogenesis of the M. cymbalaria.