Histopathological Degeneration of Spermatogenesis and Histomorphometric Alterations of the Testicular Microanatomy of Male Wistar Rats after Oral Lead Intoxication
Artículo
| IMSEAR
| ID: sea-215598
ABSTRACT
Background:
Lead is a heavy metal known to exertpathological effects on the male reproductive organs.Aim andObjectives:
To study the degeneration ofspermatogenesis and histomorphometric alterations ofthe testicular microanatomy of male Wistar rats afteroral lead intoxication. Material andMethods:
Eightymale Wistar rats were divided into four groups (1- 4)and further subdivided into 4 subgroups (A, B, C, andD) each containing five (n=5) rats depending on dosageand duration (3, 6, 9 and 12 weeks) of treatment. Group1 received 1 ml of distilled water daily. Group 2received 0.5% lead daily, Group 3 received 1.0% oflead daily and Group 4 received 1.5% of lead dailyrespectively. Testicular weights, histomorphometryand histological analysis using Toluidine Blue wereobserved.Results:
It showed that significant reductions(P<0.05) in testicular weights in the treatment groupsadministered with lead for periods of 3-9 weeks ascompared to the control while a significant increase(P<0.05) was observed in the group administered for12 weeks. Histomorphometric analysis showedsignificant reductions (P<0.05) in Seminiferous tubulediameter, length of germinal epithelium and crosssectional in the treatment groups administered forperiods of 3-12 weeks while significant increase(P<0.05) in lumen diameter was observed in thetreatment groups compared to the control.Histopathological analysis revealed that Leadinfluenced the testicular cytoarchitecture by disruptingthe processes of spermatogenesis in the treatmentgroups as compared to control.Conclusion:
The studyshowed that different dosages of lead over a period oftime had gonadotoxic effects and tendencies to altertesticular profiles by disrupting spermatogenesis.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Año:
2019
Tipo del documento:
Artículo
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