Restriction fragment length polymorphism of Mycobacterium tuberculosis strains from various regions of India, using direct repeat probe.
Artículo
en Inglés
| IMSEAR
| ID: sea-23185
ABSTRACT
Intraspecies differentiation was studied on 68 M. tuberculosis strains obtained from 6 states of India by restriction fragment length polymorphism (RFLP) using a direct repeat probe (DR probe) hybridised with Alu I digest of DNA. Most strains showed polymorphism based patterns that comprised between 2 to 7 bands and were grouped into 26 RFLP types. Of the 11 strains tested from Amritsar, 8 were RFLP type 5; the remaining 3 were of type 11 and were exclusively confined to this region. The strains from other regions were more heterogeneous. We confirm that DR-associated RFLP can be an excellent tool for the differentiation of M. tuberculosis strains. Depending on their geographical origin, these strains can be differentiated to a large extent by DR fingerprinting.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN Bacteriano
/
Secuencias Repetitivas de Ácidos Nucleicos
/
Dermatoglifia del ADN
/
Mycobacterium tuberculosis
Idioma:
Inglés
Año:
1997
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS