Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection.
Artículo
en Inglés
| IMSEAR
| ID: sea-25091
ABSTRACT
We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. The activity of this enzyme was also detected in P. falciparum culture supernatant. The parasitic origin of this enzyme was suggested by western blot analysis using anti-P. falciparum culture supernatant and anti-whole parasite antibodies. The differential inhibition of the P. falciparum GDH (NADP+) indicates that some epitopes recognised by the antibodies in both preparations may be different. The determination of P. falciparum GDH (NADP+) activity could be developed into a specific technique for the diagnosis of falciparum malaria.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Plasmodium falciparum
/
Humanos
/
Malaria Falciparum
/
Glutamato Deshidrogenasa
/
Animales
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Año:
1999
Tipo del documento:
Artículo
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