Cytotoxicity of non O1, non O139 Vibrios isolated from fresh water bodies in Vellore, south India.
Artículo
en Inglés
| IMSEAR
| ID: sea-25939
ABSTRACT
The samples of plankton, soil sediment and water from a pond, a lake and a moat respectively in and around Vellore were studied for environmental vibrios. Vibrios were isolated from all these specimens after enrichment in alkaline peptone water and subculture on selective media. Non O1, non O139 Vibrio cholerae, Aeromonas spp. and Plesiomonas spp. were isolated. There were no isolates of V. cholerae serogroup O1 and O139. Representative strains of non O1 and non O139 V. cholerae from environmental sources were tested for toxin production in Chinese hamster ovary (CHO) and Vero cell monolayer in microtitre plates. Thirty-three (91.7%) of the 36 strains tested demonstrated cytopathic effect (CPE) in both cell lines indicating their toxigenicity. PCR done on representative strains of non O1 and non O139 V. cholerae showed that none of the strains were positive for ctx A, tcp A-E and tcp C genes. These results indicate that these non agglutinating environmental vibrios produced cytotoxins other than the cholera toxin.
Texto completo:
Disponible
Índice:
IMSEAR (Asia Sudoriental)
Asunto principal:
Plancton
/
Microbiología del Suelo
/
Toxinas Bacterianas
/
Vibrio cholerae
/
Microbiología del Agua
/
Células Vero
/
Serotipificación
/
Supervivencia Celular
/
Chlorocebus aethiops
/
Cricetinae
País/Región como asunto:
Asia
Idioma:
Inglés
Año:
1999
Tipo del documento:
Artículo
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